Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro

Zhen-Xing Zhang, Li-Xue Guan, Kun Zhang, Shouxian Wang, Pei-Cheng Cao, Yong-He Wang,     Zhe Wang, Long-Jun Dai: Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro. Cell Biology International. 31:645-648, 2007.

PDF Download paper

Abstract This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.

This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and
after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials.
Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques.
All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were
not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.
 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

Bookmark and Share

Tags: , , , , ,



This entry was posted on Thursday, September 17th, 2009 at 6:29 pm and is filed under Stem Cells. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.

Leave a Reply