Mechanisms of amiloride stimulation of Mg2+ uptake in immortalized mouse distal convoluted tubule cells

    Dai LJ, Raymond L, Friedman PA, and Quamme GA: Mechanisms of amiloride stimulation of  Mg2+ uptake in immortalized mouse distal convoluted tubule cells. American Journal of Physiology 272:F249-F256, 1997.

    PDF Download paper

    Abstract The distal convoluted tubule reabsorbs – 10% of the filtered magnesium, which is -70% of that delivered to it from the loop of Henle. The cellular mechanisms of magnesium transport in the distal convoluted tubule are not known. Amiloride has been reported to promote magnesium conservation. Studies were performed on immortalized mouse distal convoluted tubule (MDCT) cells to characterize distal magnesium transport and the effects of amiloride. Intracellular free Mg”+ concentration ( [Mg2+]i) was determined on single MDCT cells using microfluorescence with mag-fura 2. Basal [Mg”+]i was 0.53 t 0.01 mM, which is -2% of the total cellular magnesium. To assess Mg2+ uptake, MDCT cells were first Mg2+ depleted (0.22 t 0.01 mM) by culturing in Mg2+-free media for 8-16 h and then placed in 5 mM MgC12, and the [Mg2+]i was determined. [Mg2+]i returned to basal levels (0.50 t 0.04 mM) with refill rate, d([Mg2+]i)ldt, of 181 t 33 r&I/s. Mg2+ entry rate was concentration dependent; a concentration of -0.1 mM resulted in half-maximal uptake rates. Mg”+ uptake was inhibited by La3+ (36 t 17 nM/s), Mn2+ (56 t 25 r&I/s), and nitrendipine (52 t 18 nM/s), but not Ca2+ (225 t 70 nnlvs). Mg2+ uptake was influenced by the transmembrane voltage; hyperpolarization either with the addition of valinomycin or the substitution of bath NaCl with NaSCN stimulated Mg2+ influx (205 ? 3 and 561 t 54 r&I/s, respectively). Depolarization with external KC1 diminished Mg2+ uptake (57 t 25 r&I/s). These data provide evidence for novel Mg2+ entry pathways in MDCT cells that are specific for Mg2+ and activated by an increase in transmembrane voltage. Because amiloride leads to a hyperpolarization of the apical membrane, we postulated that amiloride may enhance Mg2+ transport by influencing the membrane voltage. Amiloride (50 pM) increased Mg2+ uptake (235 t 79 nM/s) in a concentration.-dependent manner (half-maximal concentration of 35 uM amiloride). Accordingly, the distal diuretic, amiloride, inhibits Na+ transport, hyperpolarizes the apical membrane, and results in a stimulation of Mg2+ uptake in MDCT cells. These results provide the cellular basis for the clinical use of amiloride to bring about renal magnesium conservation.

    distal convoluted tubule reabsorbs – 10%
    of the filtered magnesium, which is -70% of that delivered to
    it from the loop of Henle. The cellular mechanisms of magnesium
    transport in the distal convoluted tubule are not known.
    Amiloride has been reported to promote magnesium conservation.
    Studies were performed on immortalized mouse distal
    convoluted tubule (MDCT) cells to characterize distal magnesium
    transport and the effects of amiloride. Intracellular free
    Mg”+ concentration ( [Mg2+]i) was determined on single MDCT
    cells using microfluorescence with mag-fura 2. Basal [Mg”+]i
    was 0.53 t 0.01 mM, which is -2% of the total cellular
    magnesium. To assess Mg2+ uptake, MDCT cells were first
    Mg2+ depleted (0.22 t 0.01 mM) by culturing in Mg2+-free
    media for 8-16 h and then placed in 5 mM MgC12, and the
    [Mg2+]i was determined. [Mg2+]i returned to basal levels
    (0.50 t 0.04 mM) with refill rate, d([Mg2+]i)ldt, of 181 t 33
    r&I/s. Mg2+ entry rate was concentration dependent; a concentration
    of -0.1 mM resulted in half-maximal uptake rates.
    Mg”+ uptake was inhibited by La3+ (36 t 17 nM/s), Mn2+
    (56 t 25 r&I/s), and nitrendipine (52 t 18 nM/s), but not Ca2+
    (225 t 70 nnlvs). Mg2+ uptake was influenced by the transmembrane
    voltage; hyperpolarization either with the addition
    of valinomycin or the substitution of bath NaCl with
    NaSCN stimulated Mg2+ influx (205 ? 3 and 561 t 54 r&I/s,
    respectively). Depolarization with external KC1 diminished
    Mg2+ uptake (57 t 25 r&I/s). These data provide evidence for
    novel Mg2+ entry pathways in MDCT cells that are specific for
    Mg2+ and activated by an increase in transmembrane voltage.
    Because amiloride leads to a hyperpolarization of the
    apical membrane, we postulated that amiloride may enhance
    Mg2+ transport by influencing the membrane voltage. Amiloride
    (50 pM) increased Mg2+ uptake (235 t 79 nM/s) in a
    concentration.-dependent manner (half-maximal concentration
    of 35 uM amiloride). Accordingly, the distal diuretic,
    amiloride, inhibits Na+ transport, hyperpolarizes the apical
    membrane, and results in a stimulation of Mg2+ uptake in
    MDCT cells. These results provide the cellular basis for the
    clinical use of amiloride to bring about renal magnesium
    conservation.
    mag-fura 2; intracellular free magnesium

    Bookmark and Share

    Tags: , , , , , ,



    This entry was posted on Friday, September 11th, 2009 at 9:05 pm and is filed under Nephrology. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.

    Leave a Reply