Dr. Long Jun Dai

Zhen-Xing Zhang, Yan-Zhen Zhang, Li-Xue Guan, Yan-Ju Zhou, Xin-Xiang Zhao, Yao-Hong Cai, Li-Mei Zhao, Feng-Qi Li, Long-Jun Dai:  Autotransplantation of purified mesenchymal stem cells in a patient with acute myocardial infarction: A case study. Journal of Stem Cells 2(3):185-189, 2007.

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Abstract Purified bone marrow-derived mesenchymal stem cells were autotransplanted to a patient with acute myocardial infarction. The employment of the sub-population of bone marrow-derived mononuclear cells was nitended to clarify some disputable outcomes in hetrogeneous mononuclear cell therapy. The improvement of myocardial perfusion and cardiac function was observed after delivery of mesenchymal stem cells through a combined procedure of primary intracoronary infusion and secondary intraveneous infusion. This procedure is expected to enhance the engraftment efficacy of transplanted cells at infarcted myocardium.

Zhen-Xing Zhang, Li-Xue Guan, Kun Zhang, Shouxian Wang, Pei-Cheng Cao, Yong-He Wang,     Zhe Wang, Long-Jun Dai: Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro. Cell Biology International. 31:645-648, 2007.

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Abstract This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.