Dr. Long Jun Dai

Dong-Sheng Li, Ya-Hong Yuan, Hang-Jun Tu, Qing-Le Liang, Long-Jun Dai: A protocol for islet isolation from mouse pancreas. Nature Protocols. 4:1649-1652, 2009.

Download Paper

Abstract The recent era of islet transplantation research began more than four decades ago. In 1967, Lacy’s group described a new collagenase-based method to isolate islets, paving the way for future islet experiments both in vitro and in vivo, and eventually leading to a successful islet transplantation to treat patients with type 1 diabetes; since then it has been referred to as the Edmonton protocol. Islet isolation has been a fundamental technique in the field of diabetic research for almost half a century. Achieving good islet isolation is one of the most important factors for reliable islet transplantation studies.

Warnock GL, Meloche RM, Thompson D, Shapiro RJ, Fung M, Ao Z, Ho S, He Z, Dai LJ, Young  L, Blackburn L, Kozak S, Kim PT, Al-Adra D, Johnson JD, Liao YH, Elliott T, Verchere CB:  Improved human pancreatic islet isolation for prospective cohort study of islet transplantation vs best medical therapy in type 1 diabetes mellitus. Archives of Surgery 140(8):735-744, 2005.

Download paper

Abstract A local multiorgan donor pancreas procurement program can provide a source for optimized isolation of purified viable islets for transplantation into patients with type 1 diabetes mellitus receiving best medical therapy.