Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro

greedy — Thu, 17 Sep 2009 22:29:25 +0000

Zhen-Xing Zhang, Li-Xue Guan, Kun Zhang, Shouxian Wang, Pei-Cheng Cao, Yong-He Wang, Zhe Wang, Long-Jun Dai: Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro. Cell Biology International. 31:645-648, 2007.

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Abstract This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.

This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and

after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials.

Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques.

All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were

not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.

Dr. Long Jun Dai » karyotypic analysis

Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro